Basic Process of PCR

PCR is an in vitro DNA replication process that uses DNA polymerases and artificial DNA primers to exponentially increase the available DNA. The thermal cycling process for PCR initially requires splitting of the DNA double helix structure. This requires a suitable DNA polymerase which is able to endure temperatures greater than 90 degrees centigrade. This process is called DNA Melting. Each strand is then cooled at a lower temperature, which is further used as a template for the production of more number of strands.