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Basic Process of PCR

PCR is an in vitro DNA replication process that uses DNA polymerases and artificial DNA primers to exponentially increase the available DNA. The thermal cycling process for PCR initially requires splitting of the DNA double helix structure. This requires a suitable DNA polymerase which is able to endure temperatures greater than 90 degrees centigrade. This process is called DNA Melting. Each strand is then cooled at a lower temperature, which is further used as a template for the production of more number of strands.

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​​"Thank you PALFIR Genetics for providing your services. I saw a documentary on delta32 and was really curious to know if I had a copy and thanks to you guys, now I know. I have one copy. Thanks Bianca for answering my questions so quickly." Adam Müller

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  • Home
  • Research
    • CCR5 Delta32
    • APOBEC3G
    • HLA-B27 and HLA-B57
    • DQB1*6 and DRB1*13
    • MTHFR C677T and A1298C
  • Procedure
    • Instructions
    • About Test
    • Testing Procedure
  • Accuracy
    • Accuracy
    • What is PCR?
    • Why PCR Testing?
    • DNA Structure and PCR
    • Basic Process of PCR
    • PCR Testing Machines
    • Uses of PCR
  • Buy Now
  • FAQ
  • Contact Us